Olympus FV3000 Inverted (#02-20)


Olympus FV3000 Inverted (#02-20)

       Overview:

  • Sensitivity: ★★★★☆
  • Speed: ★★★☆☆ (galvano), ★★★★☆ (resonant)
  • Detection channels: 2 HSD, 2 PMT, 1 Transmitted Light
  • Recommendation: Use if your sample requires higher speed than the Leica SP8 and the sample uses standard fluorophores. This is also the only confocal system with a stagetop incubator for live cell imaging and silicone lens for deep tissue imaging.

       Features:

  • Diode Lasers: 405, 445, 488, 514, 561, 640nm
  • Multidimensional imaging (XYZT)
  • Automated Macro to Micro Imaging and stitching of large area of datasets
  • Hardware Autofocus
  • NEW! Olympus X-Line objective lens for improved numerical aperture, image flatness, and chromatic correction.
  • Simultaneous Spectral Imaging
  • 2 Silicone lens for deep tissue imaging and prolong timelapse imaging
  • 2 High sensitivity detectors (HSD), 2 PMT detectors and 1 Transmitted Light Detector
  • Resonant Scanner: Fast Live Imaging (512 x 512)
  • FV31S-SW Acquisition Software
  • CellSens for Deconvolution
  • Tokai Hit on-stage Incubator (Temperature and CO2 control)
  • Other Imaging techniques: FRAP, FRET, Photostimulation, Live Imaging and Spectral Scanning
  • Excellent for Live imaging and Deep tissue Imaging

Location: #02-20

Download the instruction manual for this system here

Objective lens Magnification/N.A Working Distance (mm) Immersion Medium DIC
Olympus PlanApo N 1.25x/0.04 5.0 Dry Yes
Olympus UPlanSApo 2 10x/0.40 3.11 Dry Yes
Olympus UPlanSApo 20x/0.75 0.6 Dry Yes
Olympus UPlanXApo 40x/1.40 0.13 Oil Yes
Olympus UPlanXApo 60x/1.42 0.15 Oil Yes
Olympus UPlanXApo 100x/1.45 0.13 Oil Yes
Olympus UPlanSApo* 30x/1.05 0.8 Silicone Yes
Olympus UPlanSApo* 60x/1.3 0.3 Silicone Yes

* These objectives are not stationed in this system. The 30x silicone lens is installed in the FV3000 Upright system together with the silicone oil and the 60x silicone lens is a separate piece beside the FV3000 Inverted system in #02-20. Please borrow them and their required silicone oil from their respective locations if you need to use them, and remember to return them to their respective locations once you are done using them.


TLL Staff who would like to receive training on this system, please sign up through the Microscopy Training Form.

External users who are interested in this system, please contact the Bioimaging Group