TLL Microscopy and Imaging Facility

Below is a collection of some of the best images acquired at TLL by the different researchers.
Many featured as the Image of the month, which hang on the walls along the
Microscopy and Imaging Facility corridor.

2012

Karyogamy in Arabidopsis. F-actin pFWA::LifeAct-Venus, cytoplasm pFWA::mCherry and sperm cell nuclei pHTR10::H2B-mRFP in the central cell. Tomokazu Kawashima (Dr. Fred Berger's lab).	Magnaporthe mycelia under nitrogen starvation, visualized by TEM. Autophagic bodies accumulated in the vacuole. Deng Yi Zhen (Dr. Naweed Naqvi's lab)	Notch mutant cells in Malpighian tubules (MTs) of Drosophila melanogaster. MARCM clones , Delta protein stained with CY3 and DNA stained with Topro 3. Liu Sen (Dr. Yu Cai's lab)

Deformation of fission yeast nuclear envelope by Ba-TubZ polymers during mitosis in fission yeast. Dr. Mithilesh Mishra (Mohan Balasubramanian's Lab)
2011

Scanning electron micrograph of a mint leaf (Mentha spicata) showing the 3 types of trichomes present on it (Dr. Sarojam Rajani)	Expression of Agamous in Arabidopsis thaliana flower bud stage 4. AG::GFP and cell membrane stained with FM4-64 Wee Wan Yi (Toshiro Ito's Lab ).	Enterovirus 71, visualized by TEM. The icosahedral architecture of this 30nm virus is reflected in the hexagonal shapes, negatively stained using phospho-tungstic acid. Tanja Kiener (Jimmy Kwang's Lab ).


Fission yeast (S. pombe) expressing rlc1-3GFP (Regulatory light chain of Myosin2) with mCherry-atb2 (Tubulin) and DAPI to visualize the nucleus. Dhivya Subramanian (Mohan Balasubramanian's lab).	FACS-sorted Drosophila germ-line cell expressing Vasa (blue) and transgenic Vasa-GFP (green). The spectrosome is labeled with alpha-Spectrin antibody (red). Robyn Lim (Toshie Kai 's lab).	Drosophila testis showing the expression of the PIWI-family protein Aubergine, Vasa, and costaining for DNA. Emilie Quénerch'du (Toshie Kai 's lab).


Arabadopsis root tip showing nuclei expressing methyl transferase-CFP and cell boundaries stained with propidium iodide. Pauline Jullien (Fred Berger's lab ).
2010

Parallel arrays of microtubules at the vegetal cortex of the zebrafish embryo in early development. Tran Duc Long (Karuna Sampath's lab ).	Montage of contractile actomyosin ring formation in Schizosaccharyomyces pombe (fission yeast). Actin patches and cables are stained with phalloidin in fixed cells.Yinyi Huang (Mohan Balasubramanian’s lab).	Drosophila pupa body wall preparation. Shows nuclei with Ecdysone Receptor B1, Mical is a cytoskeletal molecule expressed throughout the neurons and Pickpocket-gal4 driven UAS-GFP. Daniel Kirilly (Yu Fengwei's lab)


HeLa cell fixed during metaphase triple lableled for Aurora B (red), microtubules (green) and DNA (blue). Image acquired on the Zeiss LSM 510 META inverted confocal and processed using the Imaris software. Vinayaka Srinivas (Maki Murata-Hori's lab)	A confocal image of a torpedo stage embryo of Arabidopsis thaliana with a chromatin CFP marker (cyan) and background autofluoresence (magenta). Image acquired using the Zeiss LSM 510. Pauline Jullien (Fred Berger's lab)	A millipede's head acquired using the SEM. Fiona Chia (Microscopy & Imaging Facility)


Two brain lobes of the Drosophila melanogaster 3rd instar larval brain. The neuroblasts are labelled with Deadpan antibody (red), while the glial cells are marked with GFP (green). The differentiated neurons are labelled with Elav antibody (blue). Serene Tan & Woo Mun Wah (Cai Yu's lab)	A lateral view of a 6 day old zebrafish with a trasposon insertion expressing an mCherry reporter in the mouth/anterior gut, lens and some head muscles. (Helen Quach Bao Ngoc & Karuna Sampath)	Neurospora crassa septation. Actin is labelled with Lifeact-GFP (green), the plasma membrane with FM4-64 (red) and the cell wall with calcofluor (blue). Meredith Calvert & Graham Wright (Balasubramanian lab)

2008-2010

An autofluorescent plant vascular bundle imaged on a Zeiss 510 Meta in 2 channels (Graham Wright)	Scanning electron micrograph of an Arabidopsis pollen grain (Annabelle Chen Siyun)	Section of a Drosophila ovary (germarium) (Daniel Kirilly) Honourable mention in Nikon's Small World Competition


Scanning electron micrograph of a cotton anther and pollen (Annabelle Chen Siyun)	Mutant follicle cells (green) expanding on the surface of fruit fly egg chamber (Daniel Kirilly) Third prize in Olympus' Bioscapes competition	Transmission electron micrograph of an endosperm cell of Jatropha curcin (Ouyang Xuezhi)


Invitorgen Molecular Probes' Fluocells slide #1 imaged on a Zeiss Exciter in 3 channels - DAPI, Alexa Fluor 488 phalloidin and Mitotracker Red CMXRos (Graham Wright)	Transmission electron micrograph of chloroplasts in a barley mesophyll cell (Ouyang Xuezhi)	"Making two organelles from one: Woronin body biogenesis by peroxisomal protein sorting" Fangfang Liu et al. (2008) Journal of Cell Biology, 180: 325-339 (Greg Jedd's lab)


An autofluorescent transverse section of Convallaria sp. imaged on a Zeiss Exciter in 2 channels (Graham Wright)	Scanning electron micrograph of an Arabidopsis meristem (Annabelle Chen Siyun)	An autofluorescent plant vascular bundle imaged on a Leica SP5 in 3 channels then rendered in Imaris (Graham Wright)


A border cell cluster in a Drosophila egg chamber. F-actin (red), DNA (blue) and Moesin-GFP in one cell of the cluster (green). Captured using the Leica SP5. De-convolved using Autoquant and 3D rendered in Imaris. (Adam Cliffe)	A zebrafish brain (3-5 day old fish), triple labelled for nuclei (blue), acetylated tubulin (axons, green), and EphB receptors (red). The image also featured in an exhibit entitled "Neuroscapes: An Exhibit in Honor of Santiago Ramon y Cajal" and in a related book. (Michael Hendricks)	Peripheral neurons (blue and green) and the nuclear ecdysone receptor (red) in a Drosophila larva. (Daniel Kirilly)


A DIC image of a freshly harvested human cheek cell (Graham's). Image acquired using a Zeiss LSM 5 Exciter, 40x 1.3 NA objective. (Graham Wright)	A confocal image of a root tip of Arabidopsis thaliana with a chromatin GFP marker (green) and propidium iodide counterstaining (red). Image acquired using the Zeiss 510 META, inverted. (Mathieu Ingouff)	An autofluorescent transverse section of Convallaria sp. imaged on a Zeiss LSM 510 in 2 channels. This composite of 12 images was captured using the motorized stage then receonstructed using ImageJ's MosaicJ plugin (Graham Wright)


The Drosophila embryonic muscles (red) with the Rolling pebbles protein (green) localized along the surface of muscles where they adhere to one another. Images were acquired using the Zeiss Meta 510 upright confocal. (Sree Devi Menon)	A montage of two pairs of Drosophila peripheral neurons labeled with RFP. Mitochondria are shown in green due to the presence of mito-GFP. (Daniel Kirilly)	An Arabidopsis thaliana root tip was stained with FM4-64, imaged in xy and z on a Zeiss LSM 510, then 3D and iso-surface rendered using Imaris. (Graham Wright)


Microtubules in the NRK (normal rat kidney epithilia) cells by immunofluorescence staining. A Z-stack was captured using the Zeiss LSM 510 META inverted confocal then projected. (Zhu Xiao Dong)	Trichomes (hairs) on the ventral surface of a Drosophila embryo which is mutant for wingless. The main component of the hair is actin, here labeled with Cy3 (red) coupled phalloidin. Green, anit-beta-catenin staining, shows the cell membranes. (Jacqueline Chin & Marita Buescher)	Inside a Winter Jasmine flower bud. Z-series of images acquired using the spinning disk confocal then the 3D volume rendered and animated using the Imaris software. (Graham Wright)


3D projection of a pollen grain, imaged on the Zeiss LSM 5 Exciter upright (Cristiana Barzaghi)	Two Arabidopsis ovules side by side that have been fertilized by pollen containing HTR12 histones labeled with GFP (green). Male HTR12 histones are expressed in the endosperm nuclei, of which the centromeres are distinguished as dots. The egg cell is labeled in red with RFP and autofluorescence of the teguments is visualized in blue. (Sarah Holec & Fred Berger)	3D surface rendering of an enucleated mammalian erythroblast. The extruded nucleus is visualized by Hoechst staining (blue), while the organization of F-actin and spectrin in the incipient reticulocyte are depicted in red and green, respectively. Z-stacks acquired using the Zeiss 510 META inverted. 3D reconstructed using Imaris software. (Tzutzuy Ramirez Hernandez)


A Drosophila melanogaster brain stained with anti-Repo antibody labelling all glia (red) and a GFP reporter line labelling a subset of glia which form the blood-brain barrier surrounding the brain (green). (Jacqueline Chin & Marita Buescher)	The tubular mitochondrial distribution (tagged with GFP) in invasive hyphae of Magnaporthe grisea (within the host rice tissue - red). The circled part shows its spread from one host cell to another through plasmodesma. The fungal hyphae are ~ 1-2 μm in diameter. (Archna Patkar & Rajesh Patkar - Naweed Naqvi's lab)	The fission yeast, Schizosaccharomyces pombe, during meiosis II. meu14-mCherry (leading edge protein; red); gfp-psy1(forespore membrane protein; green). This cell is a mutant in which two of the four forespore membranes can form and encapsulate the haploid nuclei, but two are defective and form a cup-like structure incapable of encapsulating the nuclei. (Yan Hongyan - Mohan's lab)


The developing eye imaginal disk of Drosophila melanogaster, ~3 days before eclosion. The left panel is wild-type, the right panel is a mutant in which most of the bristles, hair-like sensory organs, are missing. Red: cadherin; green: 22C10, which stains bristels. Valérie Hilgers (Cohen lab) & Smitha Vishnu (Rørth lab)	Rhodamine-labelled Taxol-stabilized microtubules bundled in vitro by purified recombinant fission yeast Transforming Acidic Coiled-Coil protein Mia1p. Rahul Thadani (Oliferenko lab)	Overexpression of a protein (green) in the fat body of a third instar Drosophila larva. Nuclear envelope is labelled with anti lamin (red), and DNA with Hoechst (blue). Sarada Bulchand Harichand (Chia lab)

This gallery will develop from the best images captured at TLL. If you have an image you would like to feature, please submit it to Meredith - you could be in with a chance of winning the 'Image of the Month' competition.

You are welcome to use these images for non-commercial purposes as long as credit is given to the person(s) who captured the image.